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97
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R&D Systems human methylcellulose complete media
Human Methylcellulose Complete Media, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems mouse methylcellulose complete media without epo
IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
Mouse Methylcellulose Complete Media Without Epo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ajinomoto Althea stemfittm basic04 ct medium
IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
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Celprogen Inc serum
IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
Serum, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Celprogen Inc incubator
IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
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Celprogen Inc mouse mesothelial cell culture complete medium
IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in <t>methylcellulose</t> medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001
Mouse Mesothelial Cell Culture Complete Medium, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in methylcellulose medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001

Journal: Nucleic Acids Research

Article Title: IRF2BP2 counteracts the ATF7/JDP2 AP-1 heterodimer to prevent inflammatory overactivation in acute myeloid leukemia (AML) cells

doi: 10.1093/nar/gkae437

Figure Lengend Snippet: IRF2BP2 depletion influences differentiation from mouse bone marrow stem and progenitor cells. ( A ) Western blot of components of the IRF2BP2 complex in cells isolated from mouse bone marrow. NUC = nucleoplasm, CHR = chromatin. ( B ) Representative bright field photography of colonies (here CFU-GM) obtained upon colony formation assays of myeloid progenitor cells with and without IRF2BP2 KD in methylcellulose medium. Additional examples are shown in . Scale = 500 μm. ( C ) Quantification of the area of colonies upon differentiation in methylcellulose. For each condition, 15 colonies were measured from two biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. ( D ) Representative bright field microscopy photography of dendritic cell-shaped cells (arrows), seen in the IRF2BP2 KD cells compared to the control cells. Scale = 50 μm. ( E ) RT-qPCR analysis of collected cells after colony formation assays. Data represents the mean of three biological replicates. Significance was evaluated via a two-tailed paired Student's t -test. ( F ) FACS quantification of surface makers after 10 days of differentiation of Lin-negative bone marrow cells in liquid culture. Data represent the mean ± s.d. of at least three biological replicates. Significance was evaluated via a two-tailed unpaired Student's t -test. Example histograms are shown in . ( G ) GSEA of TCGA and our own RNA-Seq data regarding pathways involved in myeloid leukocyte migration and stem cell maintenance. n.s. = no significant; * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The cells were seeded in duplicates at 5 × 10 3 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3, mIL-6 and mSCF (concentrations as described above), on 35 mm dishes and cultivated at 37°C and 5% CO 2 .

Techniques: Western Blot, Isolation, Two Tailed Test, Microscopy, Control, Quantitative RT-PCR, RNA Sequencing, Migration